Coding

Part:BBa_K812020:Design

Designed by: William Rostain   Group: iGEM12_Evry   (2012-09-20)


Eukaryotic PoPs -> IaaH. Indoleacetamide hydrolyase for Auxin generator, with kozak


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1025
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Kozak is included, as it cannot be added by biobrick. This part can be used to assemble biobricks for integration onto Xenopus tropicalis genome. It can also be used directly by injection into a fertilised egg, using the ready to use Xenopus plasmid BBa_K812000.


Source

This part was created from BBa_K515100, itself from P. savastanoi (By Imperial college)

References